胡 杨1，李先芝1，2*，刘 洋1，严 玲1，毛琼丽1，林 露1，杨 峰1，石 豪1
（1.劲牌有限公司，湖北大冶 435100；
2.中药保健食品质量与安全湖北省重点实验室，湖北大冶 435100）
摘要：建立HPLC法同时测定橡木桶陈酿原酒中没食子酸、香草酸、丁香酸、香草醛、丁香醛、鞣花酸6种醛酸类化合物的含量。以小曲清香型白酒为基酒，通过橡木桶进行陈酿，检测酒样中 6 种醛酸类化合物的含量。采用Waters Atlantis T3 色谱柱（4.6 mm×250 mm，5 μm），乙腈-0.1%磷酸作为流动相梯度洗脱，流速 1.0 mL/min，柱温30 ℃，检测波长270 nm；进样量10 μL。实验结果表明没食子酸、香草酸、丁香酸、香草醛、丁香醛、鞣花酸在各自范围内线性关系良好（r≥0.999 9），平均加标回收率96.07%～100.95%，RSD 0.82%～4.13%。该方法操作简单，准确度高，重复性好，为橡木桶在白酒行业中的应用提供参考依据。
关键词：白酒；橡木桶；醛酸类化合物；HPLC
中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2021）06-0104-0005
Simultaneous Determination of Multiple Index Contents in Oak Barrel Aged Raw Wine by HPLC
HU Yang1, LI Xian-zhi1，2*, LIU Yang1, YAN Ling1, MAO Qiong-li1, LIN Lu1, YANG Feng1, SHI Hao1
（1.Jing Brand Co., Ltd., Daye Hubei 435100, China;
2.Hubei Provincial Key Lab for Quality and Safety of Traditional Chinese Medicine Health Food, Daye Hubei 435100, China）
Abstract：To establish an HPLC method for the simultaneous determination of gallic acid, vanillic acid, syringic acid, vanillin, syringaldehyde, and ellagic acid in 6 kinds of aldehydes in oak barrel aged wine. The Xiaoqu fresh-flavor liquor was used as the base wine and aged in oak barrels to detect the content of 6 aldehyde compounds in the wine samples. A Waters Atlantis T3 column (4.6 mm ×250 mm, 5 μm) was used, acetonitrile-0.1% phosphoric acid was used as the mobile phase for gradient elution, the flow rate was 1.0mL/min, the column temperature was 30 ℃, and the detection wavelength was 270 nm; the injection volume was 10 μL. The experimental results show that gallic acid，vanillic acid，syringic acid, vanillin, syringaldehyde, and ellagic acid had a good linear relationship in their respective ranges (r≥0.999 9). The average recovery rate was 96.07%～100.95%, and the RSD was 0.82%～4.13%. The method has simple operation, high accuracy and good repeatability, and provides a reference for the application of oak barrels in the liquor industry.
Keywords：liquor; oak barrels; aldehydic compounds; HPLC
doi：10.3969/j.issn.1674-506X.2021.06-017
收稿日期：2021-01-22
基金项目：湖北省重点研发计划（2020BBA050）
作者简介：胡杨（1993-），男，硕士，工程师。研究方向：产品质量控制。
*通信作者
引文格式：胡杨,李先芝,刘洋,等.橡木桶陈酿原酒中6种醛酸类化合物检测方法的研究[J].食品与发酵科技,2021,57(6):104-108.
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